Molecular detection of Anaplasma platys in dogs in Nineveh province, Iraq
2024
Wisam S. Hassan | Karam M. Abdulrazzaq | Qaes T. Al-Obaidi | Khoder A. Al-Azow
The present study was planned to evaluate the infection concerning Anaplasma platys in dogs for the first time in Nineveh Province, Iraq, using microscopic examination and conventional polymerase chain reaction (PCR) techniques. Eighty-one blood samples were randomly collected from dogs (31 household dogs and 50 stray dogs). Those samples were drained from dogs introduced to the Veterinary Teaching Hospital at the University of Mosul and private veterinary clinics in Nineveh province. The microscopic examination was used to check the blood films prepared for preliminary detection of morulae of A. platys within platelets; furthermore, the molecular technique was used to validate the presence of A. platys by targeting the gltA gene. In that, PCR yielded an amplification of 690 bp band size products, which was the expected size of the targeted gene. After that, these PCR amplicons were sent for sequencing to verify the presence of the pathogen, and then the sequence results were subsequently aligned with other strains available on the NCBI database. Finally, a phylogenetic tree for the related strains was constructed by Mega-11 software. The outcomes indicated that the overall infection rate of A. platys infection in dogs was 6/81 (7.4%) and 11/81 (13.6%) using microscopic examination and molecular techniques, respectively. Additionally, there was no significant difference within the dogs' categories in this study. In conclusion, it has been shown that A. platys in dogs were circulating in Nineveh province; therefore, plans for control measures are advised.
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