Influence of the number of times for inoculum preparation, the length of preculture period and the material of culture bottles on wood-decay ability of test fungi for the laboratory decay test
2019
Kambara, K. (Forestry and Forest Products Research Institute (Japan)) | Matsunaga, H. | Ohmura, W.
The laboratory decay test specified by the Japanese Industrial Standard JIS K 1571 : 2010 defines the performance requirements for wood preservatives and the validity criterion of the test based on the mean mass loss of untreated specimens (Sugi sapwood) for each test fungus. In this study, we examined the influence of the following three culture conditions on the wood-decay ability of the test fungi for the purpose of selecting culture conditions that stably achieve the validity criterion : (1) the number of times for inoculum preparation, (2) the length of preculture period in culture bottles and (3) the material of culture bottles. We also investigated the wood-decay ability of the test fungi subcultured in different organizations. The results showed that the mean mass loss of Fomitopsis palustris almost exceeded the validity criterion of 30% among the three culture conditions. However, the wood-decay ability of F. palustris was appeared to be different between the organizations. On the other hand, Trametes versicolor apparently showed different mean mass loss with different length of preculture period. It is suggested that the mean mass loss of T. versicolor meets the requirement for validity criterion of 15%, once we set the longest preculture period of 15 days allowed by the JIS.
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