Method for nondestructive measurement of β-cryptoxanthin in satsuma mandarins (Citrus Unshiu Marcow.) using visible and near-infrared spectroscopy
2019
Hisanaga, A. (NARO Institute of Fruit and Tea Science, National Agriculture and Food Research Organization (NARO), Shimizu, Shizuoka (Japan). Okitsu Citrus Research Station) | Sakanaka, T. | Yoshioka, T. | Sugiura, M.
Satsuma mandarins (Citrus unshiu Marcow.) are one of the most commonly eaten domestic fruits in Japan, and recent studies have shown that the β-cryptoxanthin present in Satsuma mandarins might provide benefits to bone health. In this study, we examined whether the use of visible and near-infrared (VIS-NIR) spectroscopy, and a new simplified measuring system for β-cryptoxanthin in satsuma mandarins, which has recently been developed as an alternative method of HPLC, permit us to rapidly quantify β-cryptoxanthin in single intact fruits. Satsuma mandarins, harvested in 2016, were subjected to VIS-NIR radiation, and spectra were collected from 600-970nm at 1-nm intervals, with an integration time of 10ms. β-cryptoxanthin contents of each sample were then measured by a newly developed simplified measuring system. We then examined the associations between VIS-NIR spectra data and β-cryptoxanthin contents obtained from the simplified measuring system. Calibration models were prepared using partial least squares regression (PLSR) on pre-treated spectra in the ranges 0.52-3.44mg/100g (β-cryptoxanthin) of 340 samples. External validation was carried out with 340 new samples. The PLSR models for intact satsuma mandarins were able to predict β-cryptoxanthin with R**2=0.897, RMSEP=0.169, and RPD=3.01. The results allow for the market valorization of this fruit.
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