Detergent effect on cytochrome b559 electron paramagnetic resonance signals in the photosystem II reaction centre
2003
Yruela Guerrero, Inmaculada | García Rubio, Inés | Roncel Gil, Mercedes | Martínez, Jesús I. | Ramiro Pascual, María Victoria | Ortega Rodríguez, José María | Alonso, Pablo J. | Picorel Castaño, Rafael | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Detergent effect on Cytochrome b559 from spinach photosystem II was studied by electron paramagnetic resonance (EPR) spectroscopy in D1-D2-Cyt b559 complex preparations. Various n-dodecyl-β-D-maltoside concentrations from 0 to 0.2% (w/v) were used to stabilise the D1-D2-Cyt b559 complexes. Low spin heme EPR spectra were obtained but gz feature positions changed depending on detergent conditions. Redox potentiometric titrations showed a unique redox potential cytochrome b559 form (E ‘m = +123-150 mV) in all the D1-D2-Cyt b559complex preparations indicating that detergent does not affect this property of the protein in those conditions. Similar effect on Cytochrome b559 EPR spectrum was observed in more intact photosystem II preparations independently of their aggregation state. This finding indicates that changes due to detergent could be a common phenomenon in photosystem II complexes. Results are discussed in terms of the environment each detergent provides to the protein.
Afficher plus [+] Moins [-]Dirección General de Investigación Científica y Técnica PB98-1632 PB97-1135
Afficher plus [+] Moins [-]Diputación General de Aragón P17/98 P111/2001
Afficher plus [+] Moins [-]Mots clés AGROVOC
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