In-vivo modification of Azotobacter-chroococcum glutamine-synthetase
1994
Cejudo Fernández, Francisco Javier | Muñoz Centeno, María de la Cruz | Aneque Guerrero, Antonio | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | Universidad de Sevilla. Departamento de Genética
against glutamine synthetase of the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 immunoreacted with glutamine synthetase from the N2-fixing heterotrophic bacterium Azotobacter chroococcum. In Western-blotting experiments this antibody recognized a single protein of a molecular mass of 59 kDa corresponding to glutamine synthetase subunit. This protein was in vivo-labelled in response to addition of ammonium, both [3H]adenine and H332PO4 preincubation of the cells being equally effective. Nevertheless, the amount of glutamine available nitrogen source. Modified, inactive glutamine synthetase was re-activated by treatment with snake-venom phosphodiesterase but not by alkaline phosphatase. LMethionine-DL-sulphoximine, an inhibitor of glutamine synthetase, prevented the enzyme from being covalently modified. We conclude that, in A. chroococcum, glutamine synthetase is adenylylated in response to ammonium and that for the modification to take place ammonium must be metabolized.
Afficher plus [+] Moins [-]Informations bibliographiques
Cette notice bibliographique a été fournie par Universidad de Sevilla
Découvrez la collection de ce fournisseur de données dans AGRIS
