Detection of Paenibacillus larva DNA from soil samples collected in apiaries in Yamanashi Prefecture
2015
Ushlyama, K. (Eastern Livestock Hygiene Service Center, Yamanashi Prefecture, Fuefuki-shi, Yamanashi Prefecture (Japan)) | Ninomiya, U.
Spores of American foul brood (Paenibacillus larvae) have been detected from soil samples collected from apiaries in Yamanashi Prefecture by DNA fingerprinting method based on PCR. The detection limit is at 10 cfu/g in 1 g soil sample, and in the present study 20 of 67 soil samples (30%) were positive to P. larvae. Spores of P. larvae in apiary soil can be brought back to hives through water collection by honeybees, and it raises the risk of infection to American foul brood. The pasteurization of soil is not very effective, there must be a measure as if the relocation of aparies to prevent the future infection.
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