Development of a microbial detection method using the water-soluble tetrazolium salt WST: Food industry applications
2015
Tsukatani, T. (Fukuoka Industrial Technology Center, Kurume, Fukuoka (Japan). Biotechnology and Food Research Institute)
To determine microbial populations and their viability, methods assessing colonies formed and turbidity are generally used. However, these methods require a long incubation time. Thus, a rapid colorimetric microbial viability assay based on the reduction of the tetrazolium salt WST-8 via 2-methyl-1,4-naphthoquinone as an electron mediator was developed. The applicability of the assay for susceptibility testing of various bacteria to antibiotics, screening of antimicrobial substances, determination of water-soluble vitamins, and differentiation of Gram-positive and -negative bacteria was investigated. Good agreement was observed between results with the WST-8 colorimetric method and those obtained using conventional methods. The present assay method was superior to the conventional methods with respect to the overall rapidity, accuracy and convenience.
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