Growth of Cordyceps militaris on plant substrates supplemented with pupal powder of Thai variety of the silkworm, Bombyx mori
2015
Suraporn, S. (Mahasarakham University, Maha Sarakham (Thailand). Faculty of Science, Department of Biology)
Cordyceps is fungus that contains cordycepin, which can be used for medicine or supplementary. Under normal conditions, they infect and grow on insects, especially from the order Lepidoptera, but for commercial purposes, development of Cordyceps media based on locally abundant plant substrates is warranted. Here we investigated various fungal media based on different plant substrates supplemented with pupal powder obtained from Thai silkworm, Bombyx mori. Four strains of Cordyceps militaris (17944, 17991, 18247, 27815) and Paecilomyces tenuipes were grown on sticky rice, black rice, brown rice, rice, rice bran, corn, taro, soybean, and peanut, each mixed with 5 g of pupal powder at 24-25 degC, 80-90 % relative humidity. Beauveria bassiana was used as positive control. Effective recipes for mycelial growth all types of rice with pupal powder, but not in any of other plant substrates with pupal powder. The largest growth of mycelium was observed for C. militaris 17944. P. tenuipes only produced stroma when it was grown on sticky rice mixed with pupal powder and on dead silkworm pupae. Growth of stroma was not observed for C. militaris.
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