Anti-adipogenic activity of berberine in 3T3-L1 pre-adipocytes cultured in high-glucose medium
2016
Shinmoto, H. (Tamagawa University, Machida, Tokyo (Japan). School of Agriculture) | Yamaguchi, Y. | Ito, M. | Hoshizaki, R. | Naganawa, Y.
Mouse 3T3-L1 pre-adipocytes were cultured in low- and high-glucose Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FCS, and were induced to adipocytes in the presence of insulin, or insulin and berberine. While glucose was consumed within 2 days of culture in low-glucose medium, significant concentrations of glucose were observed in high-glucose medium. Berberine was also shown to enhance glucose consumption. Cellular glycerol-3-phosphate dehydrogenase (GPDH) activity and triglyceride accumulation of differentiated 3T3-L1 cells in high-glucose medium were higher than those in low-glucose medium. Berberine showed strong antiadipogenic activity with decreased GPDH activity and triglyceride accumulation.
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