Development of a restriction length polymorphism combined with direct PCR technique to differentiate goose and muscovy duck parvoviruses
2016
Wan, C.H. ((Fujian Academy of Agricultural Science/Fujian Animal Disease Control Technology Development Center, Fuzhou (China). Institute of Animal Husbandry and Veterinary Medicine), (Fujian Agriculture and Forestry University, Fuzhou (China). College of Life Sciences)) | Chen, H.M. | Fu, Q.L. | Shi, S.H. | Fu, G.H. | Cheng, L.F. | Chen, C.T. | Huang, Y. | Hu, K.H.
A restriction fragment length polymorphism combined with direct PCR technique to differentiate goose and Muscovy duck parvoviruses (GPV and MDPV) was developed based on comparison of the NS gene of GPV and MDPV. Both GPV and MDPV genomic DNA can be amplified with 641 bp using the specific PCR primers. The PCR fragments can be cut into 463 bp and 178 bp only in the case of MDPV-derived PCR products, whereas the GPV-derived PCR products cannot. The method established in this study can be used to differentiate GPV and MDPV with high specificity and precision, by using a direct PCR kit and QuickCut enzyme, as quickly as conventional PCR.
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