Characteristics of interspecific hybrids between yamatoimo (Dioscorea polystachya Turcz. cv. Ichoimo) and Japanese yam (D. japonica Thunb.) by in vitro embryo and ovule culture
2018
Suzuki, K. | Ohara, M. | Iwasa, H. | Fukasawa, Y.
With the aim of developing superior Chinese yam (Ichoimo) cultivars, we produced interspecific hybrids between Yamatoimo (Dioscorea polystachya Turcz. cv. Ichoimo) and Japanese yam (D. japonica Thunb.), and investigated their characteristics. 1. The capsules were obtained from open-pollinated Japanese yams that were planted in or on the borders of fields where Yamatoimo were cultivated. Eight seedlings were produced from them by in vitro embryo and ovule culture. 2. Murashige and Skoog's medium, containing sucrose 30 mg/L, agar 8 g/L and Casamino acids 0.2 g/L, was used for the basic medium in both cultures. Supplementation and several combinations of NAA, IAA and BA were examined for embryo development, but they did not show any specific effectiveness. 3. The seedlings were identified, by flow cytometry, as hybrids between Yamatoimo and Japanese yam. 4. The characteristics of three hybrid strains were investigated. They clearly differed from their parents in leaf size, tuber shape, and viscosity, and showed wide variation. One strain showed higher viscosity than Japanese yam. 5. The potential was thus suggested for obtaining new Dioscorea cultivars with superior characteristics by interspecific hybridization between Yamatoimo and Japanese yam.
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