Selection of resistant tomato strains by simple inoculation method using Tomato Yellow Leaf Curl Virus infectious clone
2019
Suzuki, R. (Aichi Agricultural Research Center, Nagakute, Aichi (Japan)) | Kato, M. | Fukuta, S. | Oyabu, T. | Saka, N.
Tomato yellow leaf curl virus (TYLCV), which causes tomato leaf curl disease, is transmitted by whiteflies. TYLCV tolerant/resistant tomato cultivars have been introduced as effective countermeasures against this serious disease. In the present study, TYLCV infectious clones of the Israel strain and Israel mild strain were used to conduct inoculation tests involving TYLCV tolerant/resistant tomato cultivars/strains, the course of disease symptoms was clarified, and changes in the viral DNA load were quantified using the loop-mediated isothermal amplification method. The inoculation method with infectious clones revealed symptoms and virus growth comparable to those of a conventional inoculation method using whitefly. The results demonstrated that the method could be used for screening tolerant/resistant strains.
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