Development of microsatellite markers for an endangered fern in the Ryukyus, Plagiogyria koidzumii (Plagiogyriaceae)
2019
Kurita, K. (Kyoto University, Kyoto, Kyoto (Japan). Graduate School of Agriculture) | Kyogoku, D. | Abe, A. | Yokota, M. | Isagi, Y.
We developed 10 microsatellite markers for Plagiogyria koidzumii, a critically endangered fern species found on Iriomotejima Island in the Ryukyu Archipelago, Japan and in Taiwan. These markers showed polymorphism among 65 wild individuals from Iriomotejima Island; the number of alleles per locus was 2-14, and mean observed and expected heterozygosity in the largest population were 0.276 and 0.277, respectively. A genetic structure analysis using these markers indicated clear genetic differentiation even within the narrow geographic range (ca. 10 × 8 km) on Iriomotejima Island. These microsatellite markers should be valuable for measuring genetic diversity and comparing genetic structure within and between populations.
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