Genotypic analysis of the FDC1 polymorphism that inhibits ferulic acid decarboxylation activity in industrial yeasts
2020
Hatakeyama, A. ((Yoshinogawa Co., Ltd., Nagaoka-shi, Niigata (Japan)), (Niigata University, Niigata-shi, Niigata (Japan). Graduate School of Science and Technology)) | Watanabe, Y. | Arimoto, K. | Hara, T. | Joh, T.
In the production of sake, 4-vinyl guaiacol (4-VG) is produced by the decarboxylation of ferulic acid by ferulic acid decarboxylase from Saccharomyces cerevisiae. The inactivation of ferulic acid decarboxylase has been reported to be caused by a single nucleotide substitution (c.160A>T) within the ferulic acid decarboxylase gene (FDC1). Here, we developed a detection method for the single nucleotide polymorphism within FDC1 by a polymerase chain reaction-restriction fragment length polymorphism with a mismatched primer. This simple, rapid, and sensitive method is expected to facilitate the discrimination of the 4-VG-producing ability of yeast strains.
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