Monitoring occurrence of SARS-CoV-2 in school populations: a wastewater-based approach
2022
Castro-Gutierrez, Victor | Hassard, Francis | Vu, Milan | Leitao, Rodrigo | Burczynska, Beata | Wildeboer, Dirk | Stanton, Isobel | Rahimzadeh, Shadi | Baio, Gianluca | Garelick, Hemda | Hofman, Jan | Kasprzyk-Hordern, Barbara | Kwiatkowska, Rachel | Majeed, Azeem | Priest, Sally | Grimsley, Jasmine | Lundy, Lian | Singer, Andrew C. | Di Cesare, Mariachiara
Clinical testing of children in schools is challenging, with economic implications limiting its frequent use as a monitoring tool of the risks assumed by children and staff during the COVID-19 pandemic. Here, a wastewater-based epidemiology approach has been used to monitor 16 schools (10 primary, 5 secondary and 1 post-16 and further education) in England. A total of 296 samples over 9 weeks have been analysed for N1 and E genes using qPCR methods. Of the samples returned, 47.3% were positive for one or both genes with a detection frequency in line with the respective local community. WBE offers a low cost, non-invasive approach for supplementing clinical testing and can provide longitudinal insights that are impractical with traditional clinical testing.
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