Can the time and conditions of incubation of in vivo-derived goat embryos compromise embryonic cryosurvival?.
2024
BATISTA, R. I. T. P. | APOLINARIO, G. S. | BRAIR, V. L. | CÔRTES, L. R. | RANGEL, P. S. C. | GUIMARÃES, M. P. DE P. | FONSECA, J. F. DA | SOUZA-FABJAN, J. M. G. | RIBRIO IVAN TAVARES PEREIRA BATISTA, UNIVERSIDADE FEDERAL FLUMINENSE; GICELE SANTOS APOLINARIO, UNIVERSIDADE FEDERAL FLUMINENSE; VIVIANE LOPES BRAIR, UNIVERSIDADE DE VASSOURAS; LUANA RANGEL CÔRTES, UNIVERSIDADE FEDERAL DE VIÇOSA; PAULO SÉRGIO CERQUEIRA RANGEL, UNIVERSIDADE FEDERAL FLUMINENSE; MARIANA PEDROSA DE PAULA GUIMARÃES, UNIVERSIDADE FEDERAL FLUMINENSE; JEFERSON FERREIRA DA FONSECA, CNPC; JOANNA MARIA GONÇALVES SOUZA-FABJAN, UNIVERSIDADE FEDERAL FLUMINENSE.
Evidence suggests that the conditions after collection may compromise the survival of in vivo-derived (IVD) embryos after cryopreservation. To test this hypothesis, 150 IVD embryos (grade I and II) from 25 Alpine goats were divided into three groups after recovery on day 6 (D6; D0 = AI): P3 (n=50) – embryos maintained in a 35 mm cell culture dish with 2 mL of holding medium (HM; 301 mOsm/kg) at 37 °C for 3 h on a hotplate; P6 (n = 50) – embryos kept under the same conditions as the P3 group for 6-8 h; and T6 (n = 50) embryos maintained in cryovials containing 1 mL of HM at 37°C, for 6-8 h, in a transporter. Despite previous incubation on the transporter did not modify the survival rate after cryopreservation, when compared to incubation on the hot plate, the analysis performed revealed that the experimental condition of the T6 group is able to better maintain molecular aspects of IVD goat embryos after thawing, such as apoptotic incidence and ATP production.
Afficher plus [+] Moins [-]Edição dos resumos da 37ª Annual Meeting of the Brazilian Embryo Technology Society, 2024, Atibaia, SP.
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