Induction of rabbit casein synthesis in organ culture by tilapia prolactin and growth hormone
1981
Houdebine, Louis-Marie | Walker Farmer, Susan | Prunet, Patrick | Laboratoire de Physiologie de la Lactation ; Institut National de la Recherche Agronomique (INRA) | Hormone Research Laboratory ; University of California (UC) | Laboratoire de physiologie des poissons ; Institut National de la Recherche Agronomique (INRA)
Highly purified tilapia (Sarotherodon mossambicus) prolactin and growth hormone added to culture medium of rabbit mammary gland explants specifically stimulated casein synthesis. The synthesized casein was quantified by an immunoprecipitation and further identified by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. In addition, the lactogenic activity of the fish hormones was determined by measuring the accumulation of β-casein mRNA using a 3H-labeled DNA complementary to the β-casein mRNA as a probe in a molecular hybridization. Moreover, tilapia prolactin was able to compete slightly with [125I]prolactin for the binding to rabbit mammary gland prolactin receptor. The lactogenic activities of the tilapia prolactin and growth hormone estimated by the rabbit casein assay were about 10 and 100 times lower, respectively, than the activity of ovine prolactin, which was used as a reference.
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