Enzyme immunoassay for a urinary metabolite of 4-hydroxynonenal as a marker of lipid peroxidation
2006
Guéraud, Françoise | Peiro, Géraldine | Bernard, Hervé | Alary, Jacques | Créminon, Christophe | Debrauwer, Laurent | Rathahao-Paris, Estelle | Drumare, Marie-Francoise | Canlet, Cécile | Wal, Jean-Michel, J.-M. | Bories, Georges | Xénobiotiques ; Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT) | Commissariat à l'énergie atomique et aux énergies alternatives (CEA) | Institut National de la Recherche Agronomique (INRA) | Unité de Recherche Immuno-Allergie Alimentaire ; Institut National de la Recherche Agronomique (INRA)
International audience
Afficher plus [+] Moins [-]anglais. Free radical reactions are involved in the pathogenesis of numerous diseases, so there is a real need to develop biomarkers that reflect these reactions in vivo. 4-Hydroxy-2-nonenal (HNE) is a major product of the lipid peroxidation process that is a consequence of free radical reactions. We present here the development and validation of an enzyme immunoassay (EIA) of the major urinary metabolite of HNE, namely 1,4-dihydroxynonane-mercapturic acid (DHN-MA). EIA allowed direct measurement of DHN-MA in rat urine with good sensitivity (0.02 ng/ml) and precision (intraassay CV = 5.7%). Recovery was complete (99-102%). Cross-reactivity was very low with 1,4-dihydroxynonene and with different mercapturic acids except with one other HNE urinary metabolite. Good correlation (EIA = 0.79 x LC/MS + 14.03, r = 0.877, p < 10(-8)) was obtained between EIA and liquid chromatography/mass spectrometry (LC/MS) quantitation when analyzing urine samples of rats with different oxidative status, due to treatment with either BrCCl(3) or trinitrobenzene sulfonic acid, which are known to induce hepatic lipid peroxidation or colon inflammation, respectively.
Afficher plus [+] Moins [-]Mots clés AGROVOC
Informations bibliographiques
Cette notice bibliographique a été fournie par Institut national de la recherche agronomique
Découvrez la collection de ce fournisseur de données dans AGRIS