Intimin gene (eae) subtypes-based real-time PCR strategy for the specific detection of Shiga toxin-producing Escherichia coli serotypes O157:H7, O26:H11, O103:H2, O111:H8 and O145:H28 in cattle feces
2014
Bibbal, Delphine | Loukiadis, Estelle | Kérourédan, Monique | Peytavin de Garam, Carine | Ferré, Franck | Cartier, Philippe | Gay, Emilie | Oswald, Eric | Auvray, Frédéric | Brugère, Hubert | Physiologie et Toxicologie Expérimentales ; Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT) | Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM) ; Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS) | Interactions hôtes-agents pathogènes [Toulouse] (IHAP) ; Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT) | Laboratoire d'études et de recherches en pathologie bovine et hygiène des viandes ; Agence Française de Sécurité Sanitaire des Aliments (AFSSA) | MICrobiologie de l'ALImentation au Service de la Santé (MICALIS) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech
International audience
Afficher plus [+] Moins [-]anglais. Shiga toxin-producing Escherichia coli (STEC) belonging to serotypes O157:H7, O26:H11, O103:H2, O111:H8 and O145:H28 are known to be associated with particular subtypes of the intimin gene (eae), namely γ1, β1, ɛ, θ and γ1, respectively. This study aimed at evaluating the usefulness of their detection for the specific detection of these five main pathogenic STEC serotypes in cattle feces. Using real-time PCR assays, 58.7% of 150 fecal samples were found positive for at least one of the four targeted eae subtypes. The simultaneous presence of stx, eae and one of the five O group markers was found in 58.0% of the samples, and the five targeted stx/eae/O genetic combinations were detected 143 times. However, when taking into consideration the association between eae subtypes and O group markers, the resulting stx/eae-subtype/O combinations were detected only 46 times. The 46 isolation assays performed allowed to recover 22 E. coli strains belonging to one of the five targeted STEC serogroups. By contrast, only 2 out of 39 isolation assays performed on samples that were positive for stx, eae and an O group marker, but were negative for the corresponding eae subtype, were successful. Characterization of the 24 E. coli isolates showed that 6 were STEC, including 1 O157:H7, 3 O26:H11 and 2 O145:H28. The remaining 18 strains corresponded to atypical enteropathogenic E. coli (aEPEC). Finally, the more discriminating eae subtypes-based PCR strategy described here may be helpful for the specific screening of the five major STEC in cattle feces.
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