Estimation of bacterial use of dissolved organic nitrogen compounds in aquatic ecosystems using Biolog plates
2006
Sala, M. Montserrat | Pinhassi, Jarone | Gasol, Josep M.
6 pages, 4 figures, 1 table
Afficher plus [+] Moins [-]We demonstrated that bacterioplankton utilization of N-containing substrates, in relation to overall substrate utilization, can be assessed by calculating a nitrogen use (NUSE) index from Biolog-ECO microplate readings. The NUSE index was positively correlated with bacterial-specific aminopeptidase activity in coastal plankton samples, and it decreased after ammonia or amino acid addition to seawater cultures; this indicated that the index was sensitive to changes in N concentrations in natural samples. The index provides valuable information on dissolved organic nitrogen processing by natural bacterial assemblages, and thereby substantially expands the utility of the Biolog-ECO microplates
Afficher plus [+] Moins [-]This work was supported by the EU projects BIOHAB (EVK3-CT99-00015) and BASICS (EVK3- CT2002-00078), from the Spanish Ministery of Science and Education TEMPANO (REN2001-0588/ANT) and PROCAVIR (CTM 2004-04404-C02-01/MAR), and by a CSIC I3P postdoctoral contract financed by the Fondo Social Europeo to M.M.S.
Afficher plus [+] Moins [-]Peer reviewed
Afficher plus [+] Moins [-]Mots clés AGROVOC
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