Amplification dynamics of miniature inverted-repeat transposable elements and their impact on rice trait variability

Transposable elements (TEs) are a rich source of genetic variability. Among TEs, miniature inverted-repeat TEs (MITEs) are of particular interest as they are present in high copy numbers in plant genomes and are closely associated with genes. MITEs are deletion derivatives of class II transposons, and can be mobilized by the transposases encoded by the latter through a typical cut-and-paste mechanism. However, MITEs are typically present at much higher copy numbers than class II transposons. We present here an analysis of 103 109 transposon insertion polymorphisms (TIPs) in 738 Oryza sativa genomes representing the main rice population groups. We show that an important fraction of MITE insertions has been fixed in rice concomitantly with its domestication. However, another fraction of MITE insertions is present at low frequencies. We performed MITE TIP-genome-wide association studies (TIP-GWAS) to study the impact of these elements on agronomically important traits and found that these elements uncover more trait associations than single nucleotide polymorphisms (SNPs) on important phenotypes such as grain width. Finally, using SNP-GWAS and TIP-GWAS we provide evidence of the replicative amplification of MITEs.

RC is recipient of a Juan de la Cierva-formación contract from the Spanish Ministerio de Ciencia y Innovación. This work was supported by grants from the Spanish Ministerio de Economia, Industria y Competitividad (AGL2016-78992-R/FEDER) and Ministerio de Ciencia y Innovación (PID2019-106374RB-I00/AEI/10.13039/501100011033) to JMC. Computing resources have been provided by the Red Española de Supercomputación at the Pirineus machine (RES activity BCV-2019-2-0006).

Peer reviewed