Gene flow and hybridisation in a mixed oak forest (Quercus pyrenaica Willd.;Quercus petraea (Matts.) Liebl.) in central Spain

Valbuena-Carabaña, M.; González-Martínez, Santiago C.; Sork, V. L.; Collada, Carmen; Soto, A.; Goicoechea, P. G.; Gil, Luis

Gene flow and hybridisation in a mixed oak forest (Quercus pyrenaica Willd.;Quercus petraea (Matts.) Liebl.) in central Spain

2005

Oaks are long-standing models for the study of gene flow and hybridisation. Temperate (Quercus petraea) and sub-Mediterranean (Quercus pyrenaica) oaks coexist in central Spain, showing remarkable differences in population size and structure. Q. petraea has a scattered distribution in central Spain, where it is at one of the southernmost limits of its range, and forms low-density stands; in contrast, Q. pyrenaica is widespread in the region. We selected a mixed population of the two species (∼13 ha, 176 adults and 96 saplings) to compare the patterns of gene flow within each species and the extent of introgression between them. Using five nuclear microsatellite markers, we performed a parentage analysis and found considerable immigration from outside the stand (∼38% for Q. petraea and ∼34% for Q. pyrenaica), and estimated average seed-dispersal distances of 42 and 14 m for Q. petraea and Q. pyrenaica, respectively. Introgression between species was also estimated using our microsatellite battery. First, we developed a multivariate discriminant approach and, second, we compared our results with a widely used clustering method (STRUCTURE). Both analyses were consistent with a low level of introgression between Q. petraea and Q. pyrenaica. Indeed, only 15 adult trees, ∼8.5%, were identified as putative hybrids when both methods of analysis were combined. Hybrids may be most common in contact zones due merely to physical proximity. © 2005 Nature Publishing Group All rights reserved.

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