A Cowpea Line Has Distinct Genes for Resistance to Tobacco Ringspot Virus and Cowpea Mosaic Virus

Ponz Ascaso, Fernando; Russell, Mary L.; Rowhani, A.; Bruening, G.; Department of Energy; University of California; Ponz Ascaso, Fernando [0000-0003-0344-4363]; Rowhani, A. [0000-0001-9616-2669]; Bruening, G. [0009-0001-9740-4209]

A Cowpea Line Has Distinct Genes for Resistance to Tobacco Ringspot Virus and Cowpea Mosaic Virus

1988

5 Pág.

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A previous survey of cowpea (Vigna unguiculata) lines identified one, Arlington (USDA plant introduction number 293453), that was both resistant to the comovirus cowpea mosaic virus strain SB (CPMV) and a source of protoplasts that restrict CPMV replication. We report that the budblight strain of tobacco ringspot virus (TobRV), which infects several cowpea lines including Blackeye 5, failed to induce symptoms in Arlington cowpea seedlings or to be detected at 10–12 days after inoculation by enzyme-linked immunosorbent assay. F1 progeny of Blackeye 5 × Arlington all resisted infection by TobRV. Among F2 progeny, resistance to TobRV and to CPMV were inherited as single dominant characters, but they segregated distinctly. TobRV, as well as CPMV, interfered with the increase of the comovirus cowpea severe mosaic virus in Arlington cowpeas. Previous results showed that Arlington cowpeas possess an inhibitor of CPMV-SB proteinase. No similar activity was detected against a TobRV proteinase. Thus, the resistances of Arlington cowpeas to TobRV and to CPMV, although similar in several respects, are controlled by separate genetic loci and probably operate under distinct mechanisms.

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This research was supported by the Department of Energy Program on Biological Energy Research under grant number DE-FG03-85ER13353 and by the Agricultural Experiment Station of the University of California

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Peer reviewed

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