Aplicación de la técnica de tRFLP para evaluar las variaciones en las comunidades bacterianas del rumen asociadas a la inclusión de aceites de girasol y pescado en la dieta de ovejas | Utilization of the tRFLP technique to study the changes in rumen bacterial communities associated with the dietary addition of sunflower and fish oils in sheep

3 págs, 1 fig.-- Trabajo presentado a las XIII Jornadas sobre Producción Animal (AIDA. Zaragoza 12-13 de mayo de 2009). 3 pages, 1 figure.

[EN] Four adult cannulated Merino ewes were fed a mixed diet (35:65 forage:concentrate) for 10 days. Afterwards they received the same diet supplemented with sunflower (2%) and fish (1%) oils in order to study the effect of the lipid supplementation on the rumen microbial communities. Rumen samples were taken after 0 (day 1), 3 (day 4) and 11 (day 12) days of the lipid supplementation. DNA was extracted and analysed by tRFLP (qualitative molecular technique). An effect of the experimental treatment was observed, being samples obtained on day 12 clustered separately from initial samples (day 1). On day 4, only two animals showed a similar microbial profile than on day 12, suggesting that the time necessary for the rumen ecosystem to be adapted to the lipid supplementation may depend on the individual microbial composition. Major fragments detected by tRFLP match the abundant bacterial phyla Firmicutes and Bacteroidetes, and variations in other fragments which may correspond to bacteria involved in rumen biohydrogenation (such as those belonging to the Clostridial cluster IX or the family Lachnospiraceae) were detected. These bacteria might be responsible for variations in the milk fatty acid profile in lactating ewes.

Este trabajo ha sido financiado por el Ministerio de Ciencia e Innovación (Proyecto AGL2008-04805-C02-02) y por la Junta de Castilla y León (Proyecto CSI01B08).

Peer reviewed