Molecular characterization and expression of nanos2 and gfra1a during testicular development of the european seabass (dicentrarchus labrax l.)
2021
Prat, Francisco | Simón-Díaz, Marisol | Martos-Sitcha, Juan Antonio | Gómez Peris, A. | Ministerio de Ciencia, Innovación y Universidades (España) | Agencia Estatal de Investigación (España)
As a first step to determine the involvement of nanos2 and gfra1 in the regulation of the self-renewal and maintenance of spermatogonial stem cells (SSC) and preventing spermatogonial differentiation in the European seabass, the aim of this work was to characterize and study their expression at different stages of testicular development. One sequence of nanos2 and two sequences of gfra1 (gfra1a and gfra1b) were found in the genome of seabass by blast analysis against the NCBI whole-genome shotgun contigs (wgs) database. The sequences were confirmed by PCR using testis cDNA and specific primers designed to the respective genomic sequences. Tissue specificity analysis showed expression in all tissues studied, but nanos2 expression was higher in testis, while gfra1a was highly expressed in gonads, brain and pituitary. The expression profiles of nanos2 and gfra1a obtained by qrtPCR in the testis during the reproductive cycle, suggest that both genes can have key roles during the early stages of testicular development.
Afficher plus [+] Moins [-]The authors thank to Mr Juan José Blanco for his technical assistance. Grant RTI2018-094667-B-C21 (MCIU/AEI/FEDER,UE).
Afficher plus [+] Moins [-]Peer reviewed
Afficher plus [+] Moins [-]Informations bibliographiques
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