Morphological and molecular characterisation of mucronate isolates (M form) of Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae)
2011
Gu, Jianfeng | Wang, Jiangling | Braasch, Helen | Burgermeister, Wolfgang | Schröder, Thomas
Morphological and molecular characters of five isolates of the mucronate ('M') form of Bursaphelenchus xylophylis were compared with the round-tailed ('R') form of B. xylophylis and with B. mucronatus (European type and East Asian type). The spicules of these species (types or forms) are similar . The 'M' form of B. xylophylis is distinguished from the 'R' form of B. xylophylis by a distinct mucro at the female tail end. It differs from the European type of B. mucronatus by slightly shorter female tail mucro and position of excretory pore. It is distinguished from the East Asian type of B. mucronatus by female tail shape and shorter female tail mucro. The conventional five restriction endonucleases (Rsa I, Hae III, Msp I, Hinf I and Alu I) used for obtaining ITS-RFLP patterns of Bursephalenchus species cannot distinguish the 'M' and 'R' forms of B. Xylophylus, but the two forms can be diffentiated by use of two additional restriction endonucleases (Hpy 88 I and Hha I). The molecular phylogenetic analysis based on the sequences of D2D3 LSU rDNA, ITS1/2 region and mtCOI revealed that 'M' form of B. xylophylus is genetically closest to the 'R' form by B. xylophylus, and that their sequence divergence is small.
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