A new method for the quantification of monosaccharides, uronic acidsand oligosaccharides in partially hydrolyzed xylans by HPAEC-UV/VIS

Lorenz, Dominique M.; Erasmy, Nicole

A new method for the quantification of monosaccharides, uronic acidsand oligosaccharides in partially hydrolyzed xylans by HPAEC-UV/VIS

2016

Lorenz, Dominique M. | Erasmy, Nicole

A new method for the chemical characterization of xylans is presented, to overcome the difficulties inquantification of 4-O-methyl-alpha-D-glucuronic acid (meGlcA). In this regard, the hydrolysis behavior of xylans from beech and birch wood was investigated to obtain the optimum conditions for hydrolysis, using sulfuric acid. Due to varying linkage strengths and degradation, no general method for complete hydrolysis can be designed. Therefore, partial hydrolysis was applied, yielding monosaccharides and smallmeGlcA containing oligosaccharides. For a new method by HPAEC-UV/VIS, these samples were reductively aminated by 2-aminobenzoic acid. By quantification of monosaccharides and oligosaccharides, as well as comparison with borate-HPAEC and13C NMR-spectroscopy, we revealed that the concentrations meGlcA are significantly underestimated compared to conventional methods. The detected concentrations are 85.4% (beech) and 76.3% (birch) higher with the new procedure. Furthermore, the quantified concentrations of xylose were 9.3% (beech) and 6.5% (birch) higher by considering the unhydrolyzed oligosaccharides as well.

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