The effect of hydroalcoholic extract of olive leaf on macroscopic experimental wounds caused in red fish (Carassius auratus, Linnaeus, 1758) in Guilan province.
2024
mohammad rahanandeh | Mahsa rahanandeh | Babak Tizkar | Afshar Zoghi shalmani
The present research was conducted on Red fish (Carassius auratus, Linnaeus, 1758) in “Gilan Agricultural and Natural Resources Research Center” during Feb 2023-July 2023. In order to prepare a 20% olive leaf extract ointment, 20 g of olive leaf extract was mixed with 100 g of Oserin emulsifier. A total of 30 goldfish weighing 30-25 g were placed in two aquaria of the control and treatment groups with a volume of 1x1x2, m3 of water (25o C , pH= 7/2) with proper aeration and nutritional conditions. After creating wounds on the skin of fish in the control group, no treatment was given, but in the treatment group, 20% olive leaf extract ointment was used on the wounds once per day and continued for 30 days. The healing process of the wounds was measured using calipers at interval days of 5, 10, 15, 20, 25 and 30. The results showed that within 5-10 days after using the extract ointment on fish wounds in the treatment group, the healing process was almost 3 times faster than that of the fish in the control group (p<0/05). After creating a wound in the interval of 15 to 20 days, the process of wound healing and recovery among the fish in treatment group was much faster and the average fish recovery in treatment group was 7 times higher than the wound healing in the control group (p<0/05).
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