Production of functional bovine IL-22 in a mammalian episomal expression system

Interleukin 22 is a member of the interleukin-10 superfamily of cytokines. This protein has a dual role as an inflammatory and anti-inflammatory molecule dependent on the context. IL-22 is produced mainly by immune cells and seems to have non-hematopoietic cells as its target. In this work, we report the production of bovine IL-22 for the first time in a semi-stable expression system in mammalian cells. We showed that this recombinant IL-22 possesses biological activity in bovine macrophages infected with Mycobacterium bovis and is easy to produce in large quantities. Given its role in the defence against infections, the IL-22 produced in this work has potential applications in scientific research as well as in immunotherapy to treat diseases in cattle.

Instituto de Biotecnología

Fil: Klepp, Laura Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina

Fil: Klepp, Laura Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

Fil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Medicina; Argentina

Fil: Villafañe, Luciana María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina

Fil: Villafañe, Luciana María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina

Fil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

Fil: Malinge, Pauline. Novimmune SA. Light Chain Bioscience; Suiza

Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina

Fil: Bigi, Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina