Effect of catechin/epicatechin dietary intake on endothelial dysfunction biomarkers and proinflammatory cytokines in aorta of hyperhomocysteinemic mice
2013
Noll, Christophe | Lameth, Julie | Paul, Jean-Louis | Janel, Nathalie | Université Paris Diderot - Paris 7 (UPD7) | Nutrition Lipidique et Régulation Fonctionnelle du Coeur et des Vaisseaux (NLRFC) ; Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11) | Université Paris-Sud - Paris 11 (UP11) | Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP) | EU grant AnEUploidie; Viniflhor (Office national interprofessionnel des fruits, des legumes, des vins et de l'horticulture); Ministere de l'Agriculture, programme Vin et Sante; Pathologie et biologie vasculaires; Ministere de l'Enseignement Superieur et de la Recherche
International audience
Afficher plus [+] Moins [-]anglais. Hyperhomocysteinemia is well recognized as an independent risk factor for the development of premature atherosclerosis. Atherosclerosis, however, may be prevented by polyphenols, potent antioxidant compounds with anti-atherogenic properties. Previously, we used cystathionine beta synthase-deficient mice [Cbs (+/-)] fed a high-methionine diet-a murine model of hyperhomocysteinemia-to show that daily intake of a red wine polyphenolic extract, mainly comprised of catechin and epicatechin, has a beneficial effect on aortic expression of endothelial dysfunction biomarkers and pro-inflammatory cytokines. The aim of the present study was to understand whether catechin and epicatechin, in purified forms, have anti-atherogenic effects in hyperhomocysteinemia. Cbs (+/-) mice received 50 mu g of catechin and/or epicatechin daily in drinking water for 1 month. Plasma homocysteine (Hcy) level and aortic expression of several endothelial dysfunction biomarkers (Vcam-1, Icam-1, E-selectin, and Lox-1) and pro-inflammatory cytokines (Tnf-alpha, Il-6) were assessed. We found that both catechin and epicatechin had a beneficial effect on plasma homocysteine levels and endothelial dysfunction biomarker expression; however, only catechin had a beneficial effect on pro-inflammatory cytokine expression. Further, when both polyphenols were given, a beneficial effect was observed only on pro-inflammatory cytokine expression. Catechin seems to be a more potent anti-atherogenic compound than epicatechin in hyperhomocysteinemia and should be considered as a novel therapeutic approach against endothelial dysfunction induced by this condition.
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