GROWTH AND DEVELOPMENT OF Phomopsis diachenii Sacc. IN DIFFERENT CULTURE CONDITIONS
2012
Machowicz-Stefaniak, Zofia | Zalewska, Ewa | Król, Ewa | Kowalik, Barbara
The knowledge of life requirements of phytopathogenic fungi allows to provide the periods of their mass occurrence on plant plantations and determine the optimal conditions for their isolation and culture on artificial media. Areas of occurrence of P. diachenii on caraway plants indicate the thermophilic nature of the fungus although there is a lack of information about research on this topic. In the present study the growth and development of one-spore cultures of six isolates of P. diachenii at the temperature: 0oC, 4oC, 10oC, 16oC, 22oC, 28oC, 33oC and 38oC on Czapek-Dox medium and also the growth and development of the same isolates in eight agar media at the temperature 25°C were examined. It was shown that the optimum temperature for the growth and sporulation of mostisolates of P. diachenii and for the secretion of mature conidia from conidioma ranged 22oC to 28oC. Based on the nature of growth and development of P. diachenii on the examined media, Czapek-Dox and mineral ones as slightly acidic, and possibly malt extract should be recommend for isolation of the fungus from the plant tissue. PDA and Czapek-Dox media were considered the most suitable for diagnostic purposes due to the formation of characteristic macroscopic and microscopic features on these substrates. These substrates with fragments of carnation leaves should be recommended for the stimulation of fungal sporulation.
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