Helminth community of Nectomys squamipes naturally infected by Schistosoma mansoni in an endemic area in Brazil: a comparison of 22 years apart

Produção científica do Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios.

This project was financially supported by the National Council for Scientific and Technological Development (CNPq), Brazil [315844/2023-0], Oswaldo Cruz Institute (IOC, FIOCRUZ), the Carlos Chagas Filho Foundation for Research Support in Rio de Janeiro, Brazil [E− 26/010.001597/2019 and E− 26/210.194/2019], and the Postgraduate Program on Parasite Biology (IOC-FIOCRUZ).

Karina Varella Arpon received grants from the Coordination Office for Improvement of Higher-Education Personnel (CAPES), Brazil, under finance code 001.

Rosana Gentile received a researcher fellowship from CNPq [303643/2022-6].

Arnaldo Maldonado Júnior received a researcher fellowship from CNPq [315844/2023-0].

The municipality of Sumidouro in the state of Rio de Janeiro, southeastern Brazil, is considered an area with low endemicity of Schistosoma mansoni. In this municipality, the wild water rat Nectomys squamipes is a wild reservoir of S. mansoni. A helminth community survey was carried out on N. squamipes populations in Sumidouro from 1997 to 1999. In the present study, we compared the helminth fauna and the helminth community structure of N. squamipes with a recent survey after a 22-year time interval, considering that the prevalence of S. mansoni infection in humans remained stable and that the area showed the same environmental characteristics. Seventythree host specimens of N. squamipes collected between 1997 and 1999 and 21 specimens collected in 2021 were analyzed in this study. Seven helminth species were found in each collection period. The nematode Syphacia evaginata was recorded for the first time in N. squamipes in 2021. Syphacia venteli was the most abundant species in both periods and the most prevalent in 2021. During the period from 1997 to 1999, the most prevalent species was Hassalstrongylus epsilon. Significant differences in prevalence and abundance in relation to host sex were observed only for S. mansoni in 1997–1999. Significant differences in the abundance of the helminth species over time were observed only in Physaloptera bispiculata. Hassalstrongylus epsilon, S. venteli and S. mansoni were the dominant species in both periods. Litomosoides chagasfilhoi, Echinostoma paraensei paraensei and P. bispiculata became dominant, codominant and subordinate, respectively, over time. In conclusion, the helminth community of N. squamipes remained stable, with similar species richness, prevalence and abundance values and low betadiversity over time. The occurrence of S. mansoni in the water rat has remained stable for decades, highlighting its importance for schistosomiasis control.