Overexpression of PSTOL1-like genes increases maize root surface area and biomass under low and high phosphorus conditions.
2025
NEGRI, B. F. | MACIEL, L. S. | COELHO, A. M. | BARROS, B. DE A. | GUIMARÃES, C. T. | MAGALHAES, J. V. DE | SOUSA, S. M. DE | BARBARA F. NEGRI, UNIVERSIDADE FEDERAL DE SÃO JOÃO DEL-REI; LAIANE S. MACIEL, UNIVERSIDADE FEDERAL DE MINAS GERAIS; ANTONIO MARCOS COELHO, CNPMS; BEATRIZ DE ALMEIDA BARROS, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; JURANDIR VIEIRA DE MAGALHAES, CNPMS; SYLVIA MORAIS DE SOUSA TINOCO, CNPMS.
In maize and sorghum, candidate genes were co-localized with quantitative trait loci (QTL) associated with root morphology, dry weight, and grain yield under low P. To validate the function of these genes, the rice OsPSTOL1 (as a positive control) and its maize (ZmPSTOL3.06, ZmPSTOL8.02, and ZmPSTOL8.05_1) and sorghum (Sb07g002840, Sb03g031690, and Sb03g006765) homologs were cloned downstream of an ubiquitin promoter in the pMCG1005 vector, with the Bar gene serving as a selective marker.
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