Harnessing filamentous fungi for enzyme cocktail production through rice bran bioprocessing [DATASET]

This dataset includes 7 folders with raw data of manuscript "Dynamics of interaction and internalisation of the antifungal protein PeAfpA into Penicillium digitatum morphotypes". Each forder includes a “Readme” file (.txt files) to specificy experimental conditions for data collection.

Valorization of agri-food residues has garnered significant interest for obtaining value-added compounds such as enzymes or bioactive molecules. Rice milling by-products, such as rice bran, have limited commercial value and may pose environmental challenges. Filamentous fungi are recognized for their ability to grow on residues and for their capacity to produce large amounts of metabolites and enzymes of industrial interest. Here, we used filamentous fungi to produce enzyme cocktails from rice bran, which, due to its polysaccharide composition, serves as an ideal substrate for the growth of fungi producing cellulases and xylanases. To this end, sixteen fungal strains were isolated from rice bran and identified at the species level. The species belonged to the genera Aspergillus, Penicillium, and Mucor. The Aspergillus species displayed the highest efficiency in cellulase and xylanase activities, especially A. niger var. phoenicis and A. amstelodami. A. terreus, A. tritici, and A. montevidensis stood out as xylanolytic isolates, while P. parvofructum exhibited good cellulase activity. A. niger var. phoenicis followed by A. terreus showed the highest specific enzymatic activities of α- and β-D-galactosidase, α-L-arabinofuranosidase, α- and β-D-glucosidase, and β-D-xylosidase. Additionally, proteomic analysis of A. terreus, A. niger var. phoenicis, and P. parvofructum exoproteomes revealed differences in enzyme production for rice bran degradation. A. niger var. phoenicis had the highest levels of xylanases and cellulases, while P. parvofructum excelled in proteases, starch-degrading enzymes, and antifungal proteins. Finally, two Penicillium isolates were notable as producers of up to three different antifungal proteins. Our results demonstrate that filamentous fungi can effectively valorize rice bran by producing enzyme cocktails of industrial interest, along with bioactive peptides, in a cost-efficient manner, aligning with the circular bio-economy framework.

This work was supported by the project AGROALNEXT/2022/035 by MICIN with funding from the European Union NextGenerationEU/PRTR-C17.I1 and by Generalitat Valenciana. SG holds a Juan de la Cierva Incorporación grant (IJC2020-042749-I) funded by MCIN/AEI/ 10.13039/501100011033, “ERDF: A way of making Europe”, and ‘NextGenerationEU/PRTR’. The authors also acknowledge the Severo Ochoa Excellence Program CEX 2021-001189-S funded by MCIN/AEI and by “ERDF: A way of making Europe”.

With funding from the Spanish goverment through the "Severo Ochoa Centre of Excellence" accreditation (CEX 2021-001189-S)

1. File List: -- -- 1_Figure 1:-- -- 1-1_Figure 1_ITS:-- ITS_sequences.txt-- ITS_session_def7_clustal_ Maxim_short ( def).PDF-- ITS_session_def7_clustal_ Maxim_short ( def).pptx-- ITS_session_def7_clustal_ Maxim_short (def).emf-- ITS_session_def7_clustal_ Maxim_short (def).nwk-- -- 1-2_Figure 1B_Tubuline:-- Alignment Tubulina_ clustal.meg-- tub tree session clustal_ Maximum.PDF-- tub tree session clustal_Maximum.emf-- tub tree session clustal_Maximum.MTS-- tub tree session clustal_Maximum.png-- Tubuline_sequences.txt-- -- 1-3_Figure 1C_Calmoduline:-- Alignment Calmoduline_clustal.meg-- calmodul tree session clustal_Maximum.emf-- calmodul tree session clustal_Maximum.jpg-- calmodul tree session clustal_Maximum.MTS-- calmodul tree session clustal_Maximum.PDF-- Calmoduline_sequences.txt-- d5156753.png-- -- 1-4_ Fig1_README.txt-- -- -- 2_Figure 2:-- -- 2-1_Figure 2A_ cellulase activity:-- -- 2-1-1_Statistical analysis of cellulase activity:-- datos.sgd-- Statistical analysis.docx-- statreporter.rtf-- -- 2-1-2_Glucose_5h_45ºC_ activity.xlsx-- -- -- 2-2_Figure 2B_ xylanase activity:-- ANÁLISIS ESTADÍSTICO XILOSA 240125.docx-- datos.sgd-- statfolio.SGP-- statreporter.rtf-- Xylose_30min_45ºC.xlsx-- -- -- 2-3_Fig2_README.txt-- -- -- 3_Figure 3:-- -- 3-1_Figure 3A:-- -- 3-1-1_A. amstelodami_AM_13:-- Cellulose A. amstelodami (Day 7).JPEG-- Cellulose A. amstelodami (Day 10).JPEG-- D-glucose A. amstelodami (Day 7).JPEG-- D-xylose A. amstelodami (Day 7).JPEG-- L-arabinose A. amstelodami (Day 7).JPEG-- Maltose A. amstelodami (Day 7).JPEG-- NCS A. amstelodami (Day 7).JPEG-- Rice bran A.amstelodami (Day 7).JPEG-- Starch A. amstelodami (Day 7).JPEG-- Starch A. amstelodami (Day 10).JPEG-- Xylan A. amstelodami (Day 7).JPEG-- -- 3-1-2_A. niger var. phoenicis_RT_3:-- Cellulose A. niger var. phoenicis (Day 7).JPEG-- Cellulose A. niger var. phoenicis (Day 10).JPEG-- D- glucose A. niger var. phoenicis (Day 7).JPEG-- D-xylose A. niger var. phoenicis (Day 7).JPEG-- L- arabinose A. niger var. phoenicis (Day 7).JPEG-- Maltose A. niger var. phoenicis (Day 7).JPEG-- NCS A. niger var. phoenicis (Day 7).JPEG-- Rice bran A. niger var. phoenicis (Day 7).JPEG-- Starch A. niger var. phoenicis (Day 7).JPEG-- Starch A. niger var. phoenicis (Day 10).JPEG-- Xylan A. niger var. phoenicis (Day 7).JPEG-- -- -- 3-1-3_A. terreus_AM_39:-- Cellulose A. terreus (Day 7).JPEG-- Cellulose A. terreus (Day 10).JPEG-- D. glucose A. terreus (Day 7).JPEG-- L. arabinose A. terreus (Day 7).JPEG-- Maltose A. terreus (Day 7).JPEG-- NCS A. terreus (Day 7).JPEG-- Rice bran A. terreus (Day 7).JPEG-- Starch A. terreus (Day 7).JPG-- Starch A. terreus (Day 10).JPEG-- Xylan A. terreus (Day 7).JPEG-- Xylose A. terreus (Day 7).JPEG-- -- -- 3-1-4_P. parvofructum_AM_8:-- Cellulose P. parvofructum (Day 7).JPEG-- Cellulose P. parvofructum (Day 10).JPEG-- D-glucose P. parvofructum (Day 7).JPEG-- D-xylose P. parvofructum (Day 7).JPEG-- L-arabinose P. parvofructum (Day 7).JPEG-- Maltose P. parvofructum (Day 7).JPEG-- NCS P. parvofructum (Day 7).JPEG-- Rice bran P. parvofructum (Day 7).JPEG-- Starch P. parvofructum (Day 7).JPEG-- Starch P. parvofructum (Day 10).JPEG-- Xylan P. parvofructum (Day 7).JPEG-- -- -- 3-2_Figure 3B-- Data_Figure 3.xlsx-- Statistical analysis pNPs.docx-- -- -- 3-3_Fig3_README.txt-- -- -- 4_Figure 4:-- -- 4-1_Figure_4A-- -- 4-1-1_AM8_SA_7days_ fig 4 20240730_144248_Co:-- AM8_SA_7dias_ fig 4 20240730_144248_Co_Gel.jpg-- AM8_SA_7dias_ fig 4 20240730_144248_Co_Gel.tif-- -- 4-1-2_AM39_SA_7days_ fig 4 20240730_144701_Co:-- AM39_SA_7dias_ fig 4 20240730_144701_Co_Gel.jpg-- AM39_SA_7dias_ fig 4 20240730_144701_Co_Gel.tif-- -- 4-1-3_RT3_SA_7days_ fig 4 20240730_144918_Co:-- RT3_SA_7dias_ fig 4 20240730_144918_Co_Gel.jpg-- RT3_SA_7dias_ fig 4 20240730_144918_Co_Gel.tif-- -- -- 4-2_Figure_4B:-- Proteomics data.xlsx-- -- 4-3_Fig4_README.txt-- -- -- 5_Supp Figure S1-- -- 5-1_Figure S1_A:-- -- 5-1-1_ AM_3:-- AM_3_CYA.jpg-- AM_3_MEA.jpg-- AM_3_PDA.jpg-- AM_3_YES.jpg-- -- 5-1-2_AM_8:-- AM_8_CYA.jpg-- AM_8_MEA.jpg-- AM_8_PDA.jpg-- AM_8_YES.jpg-- -- 5-1-3_ AM_9:-- AM_9_CYA.jpg-- AM_9_MEA.jpg-- AM_9_PDA.jpg-- AM_9_YES.jpg-- -- 5-1-4_AM_13:-- AM_13_CYA.jpg-- AM_13_MEA.jpg-- AM_13_PDA.jpg-- AM_13_YES.jpg-- -- -- 5-1-5_AM_15:-- AM_15_CYA.jpg-- AM_15_MEA.jpg-- AM_15_PDA.jpg-- AM_15_YES.jpg-- -- 5-1-6_AM_27:-- AM_27_CYA.jpg-- AM_27_MEA.jpg-- AM_27_PDA.jpg-- AM_27_YES.jpg-- -- 5-1-7_AM_29:-- AM_29_CYA.jpg-- AM_29_MEA.jpg-- AM_29_PDA.jpg-- AM_29_YES.jpg-- -- 5-1-8_AM39:-- AM_39_CYA.jpg-- AM_39_MEA.jpg-- AM_39_PDA.jpg-- AM_39_YES.jpg-- -- -- 5-2_Figure S1_B:-- -- 5-2-1_RB_5.4:-- RB_5.4_CYA.jpg-- RB_5.4_MEA.jpg-- RB_5.4_PDA.jpg-- RB_5.4_YES.jpg-- -- 5-2-2_RB_9:-- RB_9_CYA.jpg-- RB_9_MEA.jpg-- RB_9_PDA.jpg-- RB_9_YES.jpg-- -- 5-2-3_RB_10:-- RB_10_CYA.jpg-- RB_10_MEA.jpg-- RB_10_PDA.jpg-- RB_10_YES.jpg-- -- 5-2-4_RB_13:-- RB_13_CYA.jpg-- RB_13_MEA.jpg-- RB_13_PDA.jpg-- RB_13_YES.jpg-- -- 5-2-5_RB_13.2:-- RB_13.2_CYA.jpg-- RB_13.2_MEA.jpg-- RB_13.2_PDA.jpg-- RB_13.2_YES.jpg-- -- -- 5-2-6_RT_1:-- Negro1.jpg-- RT_1_CYA.jpg-- RT_1_MEA.jpg-- RT_1_PDA.jpg-- RT_1_YES.jpg-- -- 5-2-7_RT_3:-- RT_3_CYA.jpg-- RT_3_MEA.jpg-- RT_3_PDA.jpg-- RT_3_YES.jpg-- -- 5-2-8_RT_4:-- RT_4_CYA.jpg-- RT_4_MEA.jpg-- RT_4_PDA.jpg-- RT_4_YES.jpg-- -- -- 5-3_FigS1_README.txt-- -- -- 6_Supp Figure S2-- -- 6-1_Gel_SDS_PAGE_Supernatants:-- 230609_RT3 2023.06.01_22.43.49_Co.jpg-- 230616_AM_3 2023.06.14_02.18.23_Co.jpg-- 230616_AM_8 2023.06.16_00.07.41_Co.jpg-- 230616_AM_9 2023.06.12_01.23.42_Co.jpg-- 230616_AM_13 2023.06.12_01.19.53_Co.jpg-- 230616_AM_27 2023.06.16_00.11.33_Co.jpg-- 230616_AM_29 2023.06.12_21.56.33_Co.jpg-- 230616_AM_39 2023.06.14_02.21.57_Co.jpg-- 230616_RT_1 2023.06.12_21.53.39_Co.jpg-- 230626_AM_13 (2º) 2023.06.18_23.36.35_Co.jpg-- Figure S2. D- Aspergillus tritici.jpg-- Figure S2. G- Aspergillus chevalieri.jpg-- Figure S2. H- Aspergillus tubingensis.jpg-- RB10 2023.06.07_22.29.45_Co.jpg-- RT4 2023.06.07_22.26.41_Co.jpg-- -- 6-2_FigS2_README.txt-- -- 7_Supp Figure S3-- FigureS3.jpg-- FigS3_README.txt-- -- -- 8_ Supplementary Data.xlsx

Peer reviewed