Hydrolysis of high concentration lignocellulose suspensions with a cumulative feeding strategy: rheometry and morphogranulometry
2014
Nguyen, Tien-Cuong | Anne-Archard, Dominique | Coma, Véronique | Cameleyre, Xavier | Lombard, Eric | To, Kim Anh | Le, Tuan | Fillaudeau, Luc | Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP) ; Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse) ; Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS) | Institut de mécanique des fluides de Toulouse (IMFT) ; Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT) | Institut Polytechnique de Bordeaux (Bordeaux INP) | Ecole Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB) | Laboratoire de Chimie des Polymères Organiques (LCPO) ; Université de Bordeaux (UB)-École Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Institut Polytechnique de Bordeaux-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) | School of Biotechnology and Food Technology ; Hanoi University of Science and Technology (HUST)
Événement(s) lié(s) : - 6. International Forum on Industrial Bioprocesses (IFIBiop); Lille (FRA) - (2014-09-07 - 2014-09-10)
Afficher plus [+] Moins [-]anglais. Bioconversion of lignocelluloses is currently a major challenge if biorefining operations are to become commonplace. The objectives of the present work were to understand and describe the evolution of physical properties of lignocellulose suspensions during enzyme-based hydrolysis reaction. Experimental set-up and methodology were developed in order to carry out a multiscale study of the lignocellulosic materials under high dry content. In-situ and ex-situ rheometry and morpho-granulometry measurements were used to investigate transfer limitations (Fig. 1). Rheological behaviour was modelled and critical concentrations (Ccrit) inducing a sharp increase of viscosity were identified with Whatman paper (WP, 35 gdm/L) and paper pulp (PP, 31 gdm/L) [1]. In a first step, hydrolysis experiments demonstrate that single dimensionless viscosity-time curves, µ*=f(t*), could be established for each substrates. Analysing hydrolysis experiments lead to assume an optimal feed rate Q* linked to the critical concentration. In a second step, cumulative feeding strategies (up to 10%w/w) were conducted for WP and PP with different ratios Q/Q*. Results report the evolution of viscosity, hydrolysis rate (Fig. 2) and mean particle size. Mixing power during suspension and hydrolysis steps are discussed as a function of hydrolysis rates.
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