SANITATION OF SWEET CHERRIES IN IN VITRO CULTURE USING CHEMOTHERAPY [OZDRAVOVÁNÍ TŘEŠNÍ V IN VITRO KULTUŘE POMOCÍ CHEMOTERAPIE]
2025
Lucie Plecitá
Sweet cherry is one of the economically important fruit species that, like many other cultivated fruit trees, are attacked by viral pathogens. One of the options for effectively eliminating viral particles from infected individuals is the application of chemotherapy in in vitro culture. The aim of the work was to eliminate Prunus dwarf virus (PDV) from three cherry genotypes selected from the experimental orchard of the VŠÚO in Holovousy. The presence of PDV, without any mixed viral infections, was detected in the plant material by ELISA testing. An aseptic in vitro culture was established from dormant shoots collected in February 2018, which, after sufficient multiplication of the plant material, was used for its own chemotherapy. The nucleoside antiviral ribavirin used in human medicine was used to treat infected explants. The concentrations of the antiviral agent were set at 40 mg·l-1 and 80 mg·l-1 and the sanitation period of 30 days. Following the sanitation treatment, explants were subcultured onto a modified MS medium containing 4 mg·l-1 BAP, where a 30-day regeneration phase took place. PDV was successfully eliminated from the majority of plants at the 40 mg·l-1 ribavirin concentration. At 80 mg·l-1 viral particles were also eliminated; however, the sensitivity of the plants to the treatment was so high that this concentration is not entirely suitable for use without prior phytotoxicity testing for individual genotypes.
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