COMPARING THE EFFECT OF MODIFICATION INNNNCULTURE MEDIUIM FOR LEISHMANIA SPECIESUSINGDIFFERENT MAMMALS
2016
Nada Noori | Amina AL-Thwani | Khdhr Mawla
The efficacy of the in vitro cultivation of promastigotes of threeLeishmania spp. weretested inmodified the biphasic Novy-MacNealNicolle (NNN) medium prepared using blood from different mammals ( human bloodgroup O , horse, donkey, goat and sheep).This study was carried out to determine which modification of NNN media will begave the best yield in the shortest time fordifferent parasite species, in order to obtaina large crop of promastigotes for experimental work and for antigen preparation.Promastigotes of the three main parasite species ,Leishmaniadonovani,Leishmaniatropica and Leishmania major, isolated from patient in Bagdad andcultuerd, at equal numbers, in the 5 different NNN preparations. At the end of the 7thday, the NNN medium using horse blood produced the greatest number ofpromastigotes for all Leishmania spp. tested, 27 ,15 ,33x106 whilst goat blood providthe poorest medium, providing culture results only for L. donovani,7x106.Humanblood group O give good results, 9.2 ,5,4X106.Donkey blood 6.5,2 ,1 X106.ThenSheep blood gave 4.5, 2.5 X106 ,This finding may be explained by the fact thatLeishmaniais a nicotinamide adenine dinucleotide (NAD) auxotrophand horseerythrocytes support NAD-dependent microorganisms
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