DIRECT DETECTION OF STREPTOCOCCUS ZOOEPIDEMICUS FROM ABORTED UTERUS OF MARES BY USING PCR TECHNIQUE
2016
Jaber Afat Alwan Al-Waily | Kadhim Hasan Abbas | Ali Mohammed Ghazi
Streptococcus zooepidemicus is one of the the main causitive opportunisticpathogen of the equine genital system and one of the secondary bacterial diseasethat causes mucosal bacterial infections . In the present study, PCR techniquewas used to detect S. zooepidemicus directly from uterine samples of infectedmares based on specific amplification superoxide dismutase (sodA) gene primersthat were designed by using the partial sequence of S.equi subsp. zooepidemicusstrain 65843 superoxide dismutase (sodA) gene, (GenBank: GU436869.1) andprimer3 plus for PCR primer design. The results showed high prevalencedetection of S. zooepidemicus (76%) positive uterine swab samples (38/50). Thisstudy was concluded that S. zooepidemicus is the one of silant pathogen thatcause metritis in mares. PCR is very fast and specific tool used to detect of S.zooepidemicus.
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