DIAGNOSTIC APPLICATION OF ELISA AND IS900 PCR USING BUFFY COAT AS A SOURCE SAMPLE FOR THE DETECTION OF Mycobacterium avium SUBSPECIES Paratuberculosis IN SUBCLINICAL CASES OF BOVINE PARATUBERCULOSIS
2018
Kawther K. jaber | Fawziah A. abdullah | Rasha M. othman
John's disease or paratuberculosis is a chronic mycobacterial infection that affectsruminants, adversely, leading to huge economic losses throughout the world. Theestimation of sero-prevalence and molecular confirmation of this disease in the cattlepopulation of south-Iraq were the objectives of this study. One of the diagnostic toolsused to detect an antibody in plasma samples was the Enzyme Linked Immuno-Sorbent Assay, indirect-ELISA was used to diagnose and estimate the sero-prevalenceof paratuberculosis in cattle. Out of 156 bovine plasma samples, 81 (51.9%) werepositive and this MAP-seroprevalence is not significantly connected to age or breedof cows (P>0.05). In this study, a PCR-based detection of IS900, distinct insertionsequences of MAP from the buffy coat of seropositive cattle (n = 81) were used as aconfirmative diagnosis. The positive PCR-based detection of IS900 was observed inanimals having high S:P% ELISA values (n=29 :35.8%).
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