MOLECULAR DETECTION OF POLYMORPHISM OF HEAT SHOCK P ROTEIN 70 (hsp70) IN THE SEMEN OF ARABI RAMS
2018
Hassan Nima Habib | Bassam Yasein Khudaier | Amad Falah Hassan
Heat shock proteins Hsp70 proteins belong to the heat shock protein family, boost cellssurvival in hostile environments, involved in many cellular biological processes. Thepolymorphism of hsp70 gene relates to the possibility of tolerating different stressconditions. This study was carried out during 1st April to 31th June 2016, 20 Arabi Rams,known fertility, 2 – 2.5 years old, from the Agricultural Research Station / College ofAgriculture / University of Basra, Iraq, were used, Semen was collected by using theartificial vagina, after training well. DNA extracted then Polymerase chain reaction(PCR)amplified sequencing, BLAST analysis and multiple sequence alignment were carried out.The results showed two haplotypes, haplotype G1 , haplotype G٢ compared with samegene in GenBank, all mutations were silent except the mutation is site 514 was missenseappeared in G1. It concluded that the Arabi rams in Iraq have new polymorphism ofhsp70 gene which can help cope with difficult environmental conditions
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