A COMPARATIVE STUDY OF CULTURE METHODS, API SYSTEM AND PCR ASSAY FOR Salmonella DETECTION ISOLATED FROM HUMAN, COWS AND POULTRY IN IRAQ
2018
Mitham S. sadiq | Rasha M. Othman
The genus Salmonella is one of the most important enteric pathogens, over the worldSalmonella Entritidis and Salmonella Typhimurium are the two most widespread serotypes thatlead to salmonellosis in human and animals and are often transferred to humans by infectedanimals and their products. The present study was conducted to compare the culture methods, API20E test and PCR assays for detection of Salmonella spp. isolated from 300 different samplescollected from various sources included healthy and infected human, cows ,chickens, chickenseggs and other sources. To achieve this goal three selective media were used included (XLD, S.S.and KIA agar) in addition to using the chromogenic salmonella media and the result of culturingwas compared with the results of API 20E test and PCR assays . We find that the using ofchromogenic media and PCR assay for detection of Salmonella spp. is more current and efficientthan using selective media and/or API 20E.
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