ANTIBACTERIAL ACTIVITY OF LAURUS NOBILUS EXTRACT AGAINST Pseudomonas aeruginosa ISOLATED FROM WOUNDS IN SHEEP AFTER FALSE WOOL SHEARIN
2020
Nawres N. Jaber | Nada S. Hadi | Moaed H. Sayhood
Although sheep shearing is considered an important and a widely used process to cut offthe sheep’s wool, false wool shearing can cause serious problems by giving a chance to growbacteria. This study is aims to identify Pseudomonas aeruginosa isolated from the inflamedwounds after false wool shearing process and to evaluate the antibacterial activity of Laurusnobilus extract against this bacteria. The results of bacterial growth showed that P. aeruginosaproduced characteristic colonies on nutrient agar with pigment pyocin and β- hemolysis on bloodagar and grew on MacConkey agar but did not ferment lactose sugar. In addition, the isolateswere positive for biofilm formation using polystyrene 96 well plate. Among 6 antibiotic agents,the highest resistance was found with novobiocin, chloramphenicol and tetracycline,respectively. Laurus nobilis extract had an antimicrobial activity against P. aeruginosa. Theresults of this study revealed that hot and cold alcoholic extracts of Laurus nobilis with MICs6.5 mg/ml, 12.5 mg/ml and 50mg/ml, respectively, were more effective than hot water extract.
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