ISOLATION AND MOLECULAR IDENTIFICATION OF MDR Pseudomonas aeruginosa FROM ANIMALS AND PATIENTS IN BASRAH PROVINCE
2020
Ghasaq K. Shamkhi | Bassam Y. Khudaier
Throughout the period from October 2018 to February 2019, 278 test samples werecollected from animals and human, (55%) animal samples which are distributed to (52.3%)swab samples were from the environment of slaughters and (47.7%) milk samples were fromcow and buffalo which collected in sterile containers. The result showed that Pseudomonaswas found in (44%) samples on pseudomonas agar distributed in (24%) samples fromslaughters, (20%) samples from milk. (45%) human samples that are distributed to (48%)swab samples were from diabetic foot patients and (52%) swab samples were from patientssuffering from burns in hospitals of Basrah province. The results showed that Pseudomonaswas found in (56%) samples on pseudomonas agar, (18%) samples from diabetic foot and(38%) samples from patients suffering from burns. 46 isolates were identified using VITEK 2Kit. 25 samples identified as Pseudomonas aeruginosa which presented (54%).Antimicrobial susceptibility testing of 31 P. aeruginosa isolates compared to 13 differentantibiotics was done by the disk diffusion method. Completely isolates were resistant to as aminimum of 8 antibiotics; they exhibited the form of the multiple resistance to theantibiotics. Thirty-eight samples were tested for 16S rRNA by conventional PCR assay, 19from animal sources and 19 from patients' sources. 18 animals and 19 patient samples weredemonstrated distinct bands with approximately 618bp corresponding for P. aeruginosa.
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