Deletion of the African Swine Fever Virus Gen <i>I196L</i> in the Georgia2010 Isolate Genome Does Not Affect Virus Replication or Virulence in Domestic Pigs
2025
Elizabeth Ramirez-Medina | Paul A. Azzinaro | Alyssa Valladares | Ediane Silva | Leeanna Burton | Leandro Sastre | Vivian O’Donnell | James J. Zhu | Douglas P. Gladue | Manuel V. Borca
African swine fever (ASF) is a lethal disease of domestic pigs that is currently challenging swine production in large areas of Eurasia and the Caribbean. The causative agent, ASF virus (ASFV), is a large, double-stranded, and structurally complex virus. The ASFV genome encodes for more than 160 proteins; however, the functions of most of them are still in the process of being characterized. Recently, ASFV gene <i>I196L</i> has been reported as being critically involved in disease production in domestic pigs. We report here that a recombinant virus derived from the Georgia 2010 isolate (ASFV-G) lacking the <i>I196L</i> gene, ASFV-G-∆I196L, had the same ability to replicate in primary cultures of swine macrophage and, when experimentally inoculated in pigs, produced a fatal form of the disease similar to that caused by the parental virulent ASFV-G. Therefore, deletion of the <i>I196L</i> gene does not significantly affect virus replication and virulence in domestic pigs of the ASFV Georgia 2010 isolate.
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