A Novel Transcription Factor of Regulating Ag-8 Biocontrol to Grapevine Crown Gall
2025
Shiyu Li | Yaping Zhang | Zhenxing Liu | Yilin Gu | Yue Bi | Jianyu Yang | Weiwei Yu | Zhuoran Li | Yuanhong Wang
Grapevine crown gall (GCG) is a major disease caused by the <i>Allorhizobium vitis</i> that causes significant losses in the grape industry. In this study, a strain of <i>Agrobacterium tumefaciens</i> (Ag-8) from soil was found to have a significant effect on grapevine crown gall. The present study was conducted to investigate the potential impact of the AraC family transcription factor <i>ata</i> (<i>Agrobacterium tumefaciens</i> AraC) on the biocontrol capacity of Ag-8 and to elucidate the potential mechanisms of its action. To this end, an <i>ata</i> deletion mutant (Δ<i>ata</i>) was constructed. It was found that the biofilm, motility, and colonization of the <i>ata</i> deletion mutant strain were significantly lower than those of the wild strain Ag-8. By analyzing the Δ<i>ata</i> transcriptome data, we focused our attention on the ABC transporter protein <i>araH</i>, and RT-qPCR showed that it was significantly down-regulated in the Δ<i>ata</i> strain. In a follow-up study, the candidate gene <i>araH</i> deletion strain (Δ<i>araH</i>) was found to have significantly lower biofilm formation, motility, and biocontrol ability than the wild-type strain Ag-8. Therefore, <i>araH</i> may be implicated as a pivotal target gene of <i>ata</i> in modulating the biocontrol capability of Ag-8. This study supports developing biocontrol strategies targeting <i>ata</i>-regulated colonization in <i>Agrobacterium</i> to enhance the sustainable management of grapevine crown gall.
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