Supplementary Materials: Biorefining Brazilian green propolis: An eco-friendly approach based on a sequential high-pressure extraction for recovering high-added-value compounds
2025
Sorita, Guilherme Dallarmi | Caicedo Chacon, Wilson Daniel | Strieder, Monique M. | Rodriguez-García, Camilo | Monteiro Fritz, Alcilene | Verruck, Silvani | Ayala Valencia, Germán | Mendiola, J. A.
S.1. Materials, S.2. Extracts characterization; Figure S1: SFE kinetic of green propolis at 4 mL min−1, 200 bar, 50 °C, and 20% CPME as cosolvent; Figure S2: Pareto chart of standardized effects (a) yield, (b) TPC, (c) ABTS, (d) DPPH, and (e) LOX. Factors (A) co-solvent, (B) temperature. α = 0.05; Table S1: Experimental design for the full-factorial design (FFD) applied to obtain the optimum process parameters (co-solvent—CPME and temperature) of the SFE of bioactive molecules from green propolis as first step of biorefinery process. Extraction pressure 20 MPa and flow 4 mL min−1 constants in all experiments; Table S2: ANOVA of the fitted. Full Factorial model for SFE yield (%) from green propolis; Table S3: ANOVA of the fitted Full Factorial model for TPC (mg GAE g−1) from green propolis; Table S4. ANOVA of the fitted Full Factorial model for ABTS (µMol TE g−1) from green propolis; Table S5: ANOVA of the fitted Full Factorial model for DPPH (µMol TE g−1) from green propolis; Table S6: ANOVA of the fitted Full Factorial model for LOX (mg QE g−1) from green propolis; Table S7. Tentatively identified compounds by GC–MS/MS analysis from SFE extracts of green propolis extracts.
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