Ex vitro rooting of micro-propagated intergeneric papaya through phloroglucinol
2023
Ram, Kalu | C, Vasugi | Thomas, Pious | M.R, Dinesh
Micropropagation techniques of papaya have been optimized worldwide. The standard rapid multiplicationtechnique of papaya, which is economically viable still needs to be improved. Although establishing in vitro cultureis easy, at the same time, in vitro rooting of papaya is still very difficult. However, in vitro rooting is costly, and theroots initiated in vitro do not have lateral branches and root hairs, making them difficult to acclimatize. Hence, theexperiments on ex vitro rooting induction of micro-propagated plantlets and acclimatization were conducted. Invitro cultures were established using shoot tips from selected advanced intergeneric hybrid seedlings. They wereinoculated onto Murashige and Skoog (MS) medium + 3% sucrose + 1 μM BAP + 1 μM GA3 + 0.1 μM IAA + 0.25%phytagel until plantlets were obtained. For ex-vitro rooting, plantlets were cultured with various concentrations ofphloroglucinol (PG) to investigate the appropriate rooting condition. Direct rooting of papaya micro-cutting intopotting medium (perlite + vermiculite + coco peat; in the ratio of 1:1:2) subsequent dipping into phloroglucinolwas more effective than in vitro rooting and led to 82% survival of micro-cutting during acclimatization. PG at1000 μM was more effective than IBA in encouraging root development. The Sachet method was found to be themost appropriate for ex vitro rooting and hardening of papaya with single-step acclimatization.
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