EFFECTS OF DIFFERENT EXTRACTION CONDITIONS ON YIELD OF ANTHOCYANIN CONTENT FROM Moringa oleifera LEAVES
2022
R. Baghya Nisha | Rita Narayanan | R. Palani Dorai | D. Baskaran | C. Valli
This study was aimed to identify the better extraction conditions for anthocyanin from fresh and dry leaves of Moringa oleifera. The variables used in this study were solvents (aqueous, ethanol, methanol) and each solvent added with acidified agents (1% citric acid, 0.1% HCl and 1% acetic acid), temperatures (40, 50, 60, 70 and 80ºC), steeping times (60, 90, 120, 150, 180 and 210 min) and pH (1, 3, 5, 7 and 9). Study showed that the anthocyanin content was higher for fresh leaves of moringa extracted with test solvent methanol acidified with 0.1% HCl. From the results, extraction at 60ºC for 120 min at pH of 3 was observed as the optimum combination for the yield of maximum anthocyanin content. However, for food purposes, moringa fresh leaves are recommended to be extracted with ethanol (acidified with 1% citric acid). The yield of anthocyanin, at optimised extraction conditions, was found to be 0.359 ± 0.0005 mg C3G/g.
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