EFFECT OF SUPPLEMENTATION OF ESTRADIOL AND GROWTH HORMONE ON IN VITRO DEVELOPMENT OF PREANTRAL FOLLICLES IN SHEEP
2022
L.S.S., Varaprasad Reddy | B.R., Naik | A.V.N., Sivakumar | B, Punyakumari | J, Suresh
The aim of this study was to investigate the effects of addition of estradiol and growth hormone on in vitro growth, maturation and antrum formation of preantral follicles (PFs’) in sheep. Preantral follicles isolated from the sheep ovarian cortical slices were cultured for six days in bicarbonate buffered tissue culture medium 199B, standard culture medium supplemented with estradiol and growth hormone during the culture period at different time points. Cumulus oocyte complexes (COCs) segregated from the follicles toward the end of six days culture in various treatments were exposed to in vitro maturation for extra 24hrs. Estradiol plus growth hormone supplementation during 0-2 days of the culture significantly (P ≤ 0.05) increased the average diameter of PFs' and supported better for antrum formation. Further the in vitro maturation (IVM) of oocytes in COCs isolated were developed to metaphase II stage at a higher rate. First two (0-2) days supplementation with estradiol plus growth hormone to TCM 199B in vitro culture medium followed by standard medium in later stages (3-6 days) supports better development of PFs' and appears to be advantageous for the development of oocytes to M-II stage.
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