Effect of dietary vitamin E and nano-selenium and stocking density on growth and activity of AMP deaminase in Rainbow trout, Oncorhynchus mykiss
2024
Alizadeh, Maryam | Salati, Amir Parviz | Shahriari, Ali | Keyvanshokooh, Saeed
The present study aimed to evaluate the effects of vitamin E, selenium nanoparticles, and their combination on growth as well as the activity of the AMP deaminase in Rainbow trout kept in high density. A total of 1275 fish with an average weight of 42.6±2.3 g were acclimatized and distributed into 15 tanks at two stocking densities (low stocking density, 20 kg m?3, and high stocking density, 80 kg m?3). The fish were divided into five experimental groups according to stocking density and diet: Control (low stocking density; basal diet), Dense (high stocking density; basal diet), Vitamin E (high stocking density; 500 mg kg?1 vitamin E supplemented diet), Nano-selenium (high stocking density; 1 mg kg?1 nano-selenium supplemented diet) and Combination (high stocking density; 500 mg kg?1 vitamin E and 1 mg kg?1 nano-selenium supplemented diet). The best growth performance and health status of fish under high stocking density conditions were observed in fish-fed vitamin E-supplemented diets (Vitamin E and combination groups). The combination of nano-selenium with vitamin E improved the performance of the fish; nano-selenium had no significant effects on the growth performance of fish under high stocking density conditions. The results showed that the stocking density did not affect the activity of AMP deaminase, since its activity did not show a significant difference between experimental groups. In addition, AMP deaminase enzyme activity did not show significant differences among groups fed with vitamin E, nano-selenium and combination.
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